NaGanoHeal represents a novel integration of traditional medicine and nanotechnology, introducing a Ganoderma-based wound healing film formulated with biocompatible polymers and nanoparticle technology. Encapsulation of Ganoderma within chitosan–hyaluronic acid nanoparticles enhances stability, enables sustained release, and improves local bioavailability. The optimized nanoparticles and film collectively ensure durability, antioxidant properties, and effective wound recovery.