Lactobacillus acidophilus, TW01 is a specific strain obtained from fermented coffee grounds in Taiwan's Gukeng region. The experiment confirmed that L. acidophilus TW01 possesses a certain level of antioxidant activity, particularly in reducing free radicals and reactive oxygen species (ROS) concentrations. It also promotes Caco-2 cell migration to cover more wound areas and maintains Th1/Th2 immune balance. Additionally, it can reduce ROS production, cell death, and immunosuppression induced b